微精蛋白β(MSMb)重组蛋白(Human,人) 

Recombinant Microseminoprotein Beta (MSMb)

MSM-B; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP57; PSP94; Immunoglobulin-binding factor; Prostate secreted seminal plasma protein; plasma beta-inhibin

[ PROPERTIES ]

Host: E.coli

Residues: Asn19~Ile114 , with two N-terminal Tags, His-tag and S-tag.

Subcellular Location: Secreted

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 16.7kDa

Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- NA

SCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC TCYETEISCC TLVSTPVGYD

KDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII

[ REFERENCES ]

1. Mbikay M., et al. (1987) DNA 6:23-29. 

2. Ulvsbaeck M., et al. (1989) Biochem. Biophys. Res. Commun. 164:1310-1315. 

3. Green C.B., et al. (1990) Biochem. Biophys. Res. Commun. 167:1184-1190. 

4. Nolet S., et al. (1991) Biochim. Biophys. Acta 1089:247-249.

[ IDENTIFICATION ]

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