胎盘钙黏蛋白(P-cadherin)重组蛋白(Mouse,小鼠2)

Recombinant Placental Cadherin (P-cadherin)

CDH3; HJMD; PCAD; Cadherin 3 Type 1; CDHP; Cadherin, Placental

[ PROPERTIES ]

Host: E.coli

Residues: Ser565~Asp822 (Accession # P10287),with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Cell membrane;Single-pass type I membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: : Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 60.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SPNSSP FQAQLTHDSDIYWMAEVSEK GDTVALSLKK FLKQDTYDLH LSLSDHGNRE QLTMIRATVC DCHGQVFNDCPRPWKGGFIL PILGAVLALL TLLLALLLLV RKKRKVKEPL LLPEDDTRDN VFYYGEEGGGEEDQDYDITQ LHRGLEARPE VVLRNDVVPT FIPTPMYRPR PANPDEIGNF IIENLKAANTDPTAPPYDSL LVFDYEGSGS DAASLSSLTT SASDQDQDYN YLNEWGSRFK KLADMYGGGEDD

[ IDENTIFICATION ]

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