β-位淀粉样前体蛋白裂解酶2(BACE2)重组蛋白 (Rat,大鼠)

Recombinant Beta Secretase 2 (BACE2)

AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP; Beta-Site APP Cleaving Enzyme 2; Aspartic-like protease 56 kDa; Aspartyl protease 1; Down region aspartic protease; Memapsin-1

[ PROPERTIES ]

Host: E.coli

Residues: Gly242~Val502,with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Membrane; Single-passtype I membrane protein. Golgi apparatus.Endoplasmic reticulum. Endosome. Cell surface

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 60.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-GGSLVLGGI EPSLYKGDIWYTPIKEEWYY QIEILKLEIG GQSLNLDCRE YNADKAIVDS GTTLLRLPQK VFDAVVEAVARTSLIPEFSD GFWTGAQLAC WTNSETPWAY FPKISIYLRD ENASRSFRIT ILPQLYIQPMMGAGFNYECY RFGISSSTNA LVIGATVMEG FYVVFDRAQR RVGFAVSPCA EIAGTTVSEISGPFSTEDIA SNCVPAQALN EPILWIVSYA LMSVCGAILL VLILLLLFPL HCRHAPRDPEVV

[ IDENTIFICATION ]

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