糖原脱支酶(AGL)重组蛋白 (Human,人)
Recombinant Glycogen Debranching Enzyme (AGL)
GDE; Amylo-1,6-Glucosidase; 4-Alpha-Glucanotransferase; Glycogen debrancher; 4-alpha-glucanotransferase; Oligo-1,4-1,4-glucantransferase; Dextrin 6-alpha-D-glucosidase
[ PROPERTIES ]
Residues: Met1167~Leu1532
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P35573
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6
Predicted Molecular Mass: 45.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH?O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MVPN GLDILKCPVS RMYPTDDSAP LPAGTLDQPL FEVIQEAMQK HMQGIQFRERNAGPQI DR NM KDEGF NI TAG VDEETGFVYG GNRF NCG TWMDKMGES DRA RNRGIPATPRD GSAVEIVGLS KSAVRWLLEL SKKNIFPYHE VTVKRHGKAI KVSYDEWNRKIQDNFEKLFH VSEDPSDLNE KHPNLVHKRG IYKDSYGASS PWCDYQLRPNFTIAMVVAPELFTTEKAWKA LEIAEKKLLG PLGMKTLDPD DMVYCGIYDN ALDNDNYNLA KGFNYHQGPEWLWPIGYFLR AKLYFSRLMG PETTAKTIVL VKNVLSRHYV HLERSPWKGL PELTNENAQYCPFSCETQAW SIATILETLY DL

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