卵泡抑素样蛋白1(FSTL1)重组蛋白(Rat,大鼠) 

Recombinant Follistatin Like Protein 1 (FSTL1)

FRP; FSL1; Follistatin-related protein 1

[ PROPERTIES ]

Host: E.coli

Residues: Glu20~Ile306

Tags: N-terminal His-Tag

Accession: Q62632

Subcellular Location: Secreted

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 34.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

E EQ RSKSKI CA NVF CGAG RE C AVTEKGEPT C LCIEQ CKP HK RPV CGS N GKTYLNHCELHRD ACLTGSKIQV DYDGHCKEKK SVSPSASPVV CYQANRDELRRRIIQWLEAEIIPDGWFSKG SNYSEILDKY FKSFDNGDSH LDSSEFLKFV EQNETAVNIT AYPNQENNKLLRGLCVDALI ELSDENADWK LSFQEFLKCL NPSFNPPEKK CALEDETYAD GAETEVDCNRCVCSCGHWVC TAMTCDGKNQ KGVQTHTEEE MTRYAQELQK HQGTAEKTKKVNTKEI

[ REFERENCES ]

1. Zwijsen A., et al. (1994) Eur. J. Biochem. 225:937-946.

2. Malik-Hall M., et al. (2003) Brain Res. Mol. Brain Res. 110:298-304.

3. Remmers E.F., et al. (1999) Transplant. Proc. 31:1549-1554.

4. Zhang G., et al. (2003) J. Neurosci. Res. 72:33-45.

[ IDENTIFICATION ]

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