彩斑4-20同源物1抑制因子2(SUV420H2)重组蛋白(Mouse,小鼠) 

Recombinant Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2)

KMT5C; Lysine N-methyltransferase 5C; Histone-lysine N-methyltransferase SUV420H2

[ PROPERTIES ]

Residues: Gly2~Leu279,with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Nucleus. Chromosome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.6

Predicted Molecular Mass: 33.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF- GPDRVTARE LCENDDLATS LVLDPYLGFR THKMNVSPVPTLRRQHHLRS ALEAFLRQRD LEAAFRALTL GGWMAHYFQS RAPRQEAALK THIFCYLRAFLPESGFTILP CTRYSMETNG AKIVSTRAWK KNEKLELLVG CIAELREEDE DLLRAGENDFSIMYSTRKRS AQLWLGPAAF INHDCKPNCK FVPSDGNTAC VKVLRDIEPGDEVTCFYGEGFFGEKNEHCE CYTCERKGEG AFRLQPREPE LRPKPLDKYE LRETKRRLQQGLVSSQQSL

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