成纤维细胞生长因子21(FGF21)重组蛋白(Human,人) 

Recombinant Fibroblast Growth Factor 21 (FGF21)

[ PROPERTIES ]

Accession: Q9NSA1

Host: E.coli

Residues: His29~Ser209

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Secreted

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 23.2kDa

Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

The sequence of the target protein is listed below.

HP IP DSSPLLQF GGQVRQRYLY TDDAQQTEAH LEIREDGTVG GAADQSPESL

LQLKALKPGV IQILGVKTSR FLCQRPDGAL YGSLHFDPEA CSFRELLLED GYNVYQSEAH

GLPLHLPGNK SPHRDPAPRG PARFLPLPGL PPALPEPPGI LAPQPPDVGS SDPLSMVGPS

QGRSPSYAS

[ IDENTIFICATION ]

[ REFERENCES ]

1. Nishimura T., et al. (2000) Biochim. Biophys. Acta 1492:203-206.
2. Clark H.F., et al. (2003) Genome Res. 13:2265-2270.
3. Zhang Z., Henzel W.J. (2004) Protein Sci. 13:2819-2824.
4. Kurosu H., et al. (2007) J. Biol. Chem. 282:26687-26695.

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