钙蛋白酶7(CAPN7)重组蛋白(Human,人) 

Recombinant Calpain 7 (CAPN7)

PalBH; Calpain Like Protease; Homolog Of Aspergillus Nidulans PALB

[ PROPERTIES ]

Residues: Met1~Pro157

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9Y6W3

Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 21.5kDa

Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MDATALERDA VQFARLAVQR DHEGRYSEAV FYYKEAAQAL IYAEMAGSSL ENIQEKITEYLERVQALHSA VQSKSADPLK SKHQLDLERA HFLVTQAFDE DEKENVEDAI ELYTEAVDLCLKTSYETADK VLQNKLKQLA RQALDRAEAL SEPLTKP

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