Thy1细胞表面抗原(CD90)重组蛋白(Mouse,小鼠) 

Recombinant Cluster of Differentiation 90 (CD90)

Thy1; CDw90; THY mocyte differentiation antigen 1

[ DESCRIPTION ]

Protein Names: Thy1 Cell Surface Antigen

Synonyms: Thy1, Thy-1

Species: Mouse

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor.

[ ROPERTIES ]

Residues: Gln20~Cys131 , with N-terminal His-Tag.

Grade & Purity: >95%, 14 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method)

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 14.3 kDa

Predicted isoelectric point: 9.0

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month

Aliquot and store at -80°C for 12 months

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCES ]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

MGHHHHHHSGSEF-QKVTSLTACLVNQNLRLDCRHENNTKDNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYFCELQVSGANPMSSNKSISVYRDKLVKC

[ IDENTIFICATION ]

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