α-胞衬蛋白(SPTAN1)重组蛋白(Rat,大鼠2) 

Recombinant Alpha-Fodrin (SPTAN1)

SPTAN1; NEAS; (ALPHA)II-SPECTRIN; Spectrin Alpha Non-Erythrocytic 1; Spectrin Alpha Chain,Brain; Spectrin, non-erythroid alpha subunit

[ PROPERTIES ]

Host: E.coli

Residues: Leu1571~Asp1712 ,with N-terminal His-Tag.

Subcellular Location: Cytoplasm, cytoskeleton. cell cortex.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 17.4kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF - LQTASDESYK DPTNIQSKHQ KHQAFEAELH ANADRIRGVI

DMGNSLIERG ACAGSEDAVK ARLAALADQW QFLVQKSAEK SQKLKEANKQ QNFNTGIKDF

DFWLSEVEAL LASEDYGKDL ASVNNLLKKH QLLEADISAH ED

[ IDENTIFICATION ]

[ REFERENCES ]

1. Hong W., Doyle D. (1989) J. Biol. Chem. 264:12758-12764. 

2. Nicolas G., et al. (2002) Mol. Cell. Biol. 22:3527-3536. 

3. Lehtonen S., et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:9814-9819. 

4. Hoffert J.D., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164.

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