α-胞衬蛋白(SPTAN1)重组蛋白(Rat,大鼠) 

Recombinant Alpha-Fodrin (SPTAN1)

SPTAN1; NEAS; (ALPHA)II-SPECTRIN; Spectrin Alpha Non-Erythrocytic 1; Spectrin Alpha Chain,Brain; Spectrin, non-erythroid alpha subunit

[ PROPERTIES ]

Host: E.coli

Residues: Trp1004~Arg1181 ,with N-terminal His-Tag

Subcellular Location: Cytoplasm, cytoskeleton,cell cortex.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 22.0kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF - WWKVEVN DRQGFVPAAY VKK LDPAQSA SRE NLLEEQGSIALRQGQID NQTRITKEAG SVSLRMKQVE ELYQSLLELG EKRKGMLEKS CKKFMLFREANELQQWINEK EAALTSEEVG ADLEQVEVLQ KKFDDFQKDL KANESRLKDI NKVAEDLESEGLMAEEVQAV QQQEVYGMMP R

[ REFERENCES ]

1. Hong W., Doyle D. (1989) J. Biol. Chem. 264:12758-12764.

2. Nicolas G., et al. (2002) Mol. Cell. Biol. 22:3527-3536.

3. Lehtonen S., et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:9814-9819.

4. Hoffert J.D., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164.

[ IDENTIFICATION ]

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