半乳糖-3-O-磺基转移酶1(GAL3ST1)重组蛋白(Human,人) 

Recombinant Galactose-3-O-Sulfotransferase 1 (GAL3ST1)

CST; Cerebroside Sulfotransferase; Galactosylceramide sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase

[ PROPERTIES ]

Residues: Leu57~Phe196,with two N-terminal Tags, His-tag and GST-tag. 

Host: E. coli

Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 8.1

Predicted Molecular Mass: 48.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAK FE R QHM DSP D LG TGG G SGI EGR GSMG YRGSEF - LEPEAVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ KHRLKFAFPN GRNDFDYPTFFARSLVQDYR PGACFNIICN HMRFHYDEVR GLVPTNAIFI TVLRDPARLF ESSFHYFGPVVPLTWKLSAG DKLTEF

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