睾丸特异性激酶1(TESK1)重组蛋白(Mouse,小鼠)
Recombinant Testis Specific Kinase 1 (TESK1)
Dual Specificity Testis-Specific Protein Kinase 1
[ PROPERTIES ]
Accession: O70146
Host: E.coli
Residues: Phe52~Ile309
Tags: Two N-terminal Tags, His-tag and T7-tag
Purity: >95%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 32.9kDa
[ USAGE ]
Reconstitute in sterile ddH?O.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
FDCAEKIGA GFFSEVYKVR HRQSGQVMVL KMNKLPSNRS NTLREVQLMNRLRHPNILRFMGVCVHQGQL HALTEYMNGG TLEQLLSSPE PLSWPVRLHL ALDIAQGLRY LHAKGVFHRDLTSKNCLVRR EDRGFTAVVG DFGLAEKIPV YREGTRKEPL AVVGSPYWMA PEVLRGELYDEKADVFAFGI VLCELIARVP ADPDYLPRTE DFGLDVPAFR TLVGNDCPLP FLLLAIHCCSMEPSTRAPFT EITQHLEQI
[ IDENTIFICATION ]

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