赖氨酸特异性去甲基化酶4A(KDM4A)重组蛋白(Human,人) 

Recombinant Lysine Specific Demethylase 4A (KDM4A)

JMJD2; JMJD2A; JHDM3A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A

[ PROPERTIES ]

Residues: Tyr141~Lys310,with two N-terminal Tags, His-tag and T7-tag. 

Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 23.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- YEKHVDEWNI GRLRTILDLVEKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL HFGEPKSWYS VPPEHGKRLERLAKGFFPGS AQSCEAFLRH KMTLISPLML KKYGIPFDKV TQEAGEFMIT FPYGYHAGFNHGFNCAESTN FATRRWIEYG KQAVLCSCRK

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