50kDa核孔蛋白(NUP50)重组蛋白(Human,人) 

Recombinant Nucleoporin 50kDa (NUP50)

NPAP60; NPAP60L; Nuclear pore-associated protein 60 kDa-like

[ PROPERTIES ]

Residues: Asn37~Ala269,with two N-terminal Tags, His-tag and T7-tag. 

Host: E. coli

Subcellular Location: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 27.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSS HH HH H H SS G LVP RGS H MASM TGG Q QM GR GS - NRAI KKA K R RNVG FESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF ASAKAAADPKVAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA CVGNAYHKQL AALNCSVRDWIVKHVNTNPL CDLTPIFKDY EKYLANIEQQ HGNSGRNSES ESNKVAAETQSPSLFGSTKLQQESTFLFHG NKTEDTPDKK MEVASEKKTD PSSLGATSA

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。