胰岛素样生长因子结合蛋白6(IGFBP6)重组蛋白(Human,人)
Recombinant Insulin Like Growth Factor Binding Protein 6 (IGFBP6)
IBP6; IGF-binding protein 6
[ PROPERTIES ]
Residues: Gly31~Gly240, with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 23.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8℃ for one month.
Aliquot and store at -80℃ for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.
MGHHHHHHSG SEF-GCGQGVQAGC PGGCVEEEDG GSPAEGCAEA EGCLRREGQECGVYTPNCAP GLQCHPPKDD EAPLRALLLG RGRCLPARAP AVAEENPKES KPQAGTARPQDVN RRDQQRN PG TST TPSQP NSAGVQ DTEM GP CRRH LDSV LQQ LQTEVY RGAQTLYVPNC DHRGFYRKRQ CRSSQGQRRG PCWCVDRMGK SLPGSPDGNGSSSCPTGSSG

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