神经调节素1(NRG1)重组蛋白(Rat,大鼠3) 

Recombinant Neuregulin 1 (NRG1)

ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor

[ PROPERTIES ]

Host: E.coli

Residues: Pro37~Thr169

Tags: N-terminal His Tag

Accession: P43322

Subcellular Location: Cell membrane;Single-pass type I membrane protein. Secreted.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 16.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.PRLK EMKSQESAAG SKLVLRCETS SEYSSLRFKW FKNGNELNRK NKPENIKIQKKPGKSELRIN KASLADSGEY MCKVISKLGN DSASANITIV ESNEFITGMP ASTETAYVSSESPIRISVST EGANTSSST

[ REFERENCES ]

1. Wen D., et al. (1994) Mol. Cell. Biol. 14:1909-1919.

2. Wen D., et al. (1992) Cell 69:559-572.

3. Peles E., et al. (1992) Cell 69:205-216.

4. Wang J.Y., et al. (1998) J. Biol. Chem. 273:20525-20534.

[ IDENTIFICATION ]

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