跨膜蛋白27(TMEM27)重组蛋白(Human,人) 

Recombinant Transmembrane Protein 27 (TMEM27)

NX17; Collectrin

[ PROPERTIES ]

Host: E.coli

Residues: Cys17~Asp137 ,with N-terminal His-Tag

Subcellular Location: Membrane; Single-passtype I membrane protein.

Purity: >95% 

Traits: Freeze-dried powder

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.9

Predicted Molecular Mass: 15.3kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-CQPG AENAFKVRLS IRTALGDKAY AWDTNEEYLF KAMVAFSMRKVPNREATEIS HVLLCNVTQR VSFWFVVTDP SKNHTLPAVE VQSAIRMNKNRINNAFFLNDQTLEFLKIPS TLAPPMD

[ REFERENCES ]

1. Zhang H., et al. (2001) J. Biol. Chem. 276:17132-17139.

2. Clark H.F., et al. (2003) Genome Res. 13:2265-2270.

3. Fukui K., et al. (2005) Cell Metab. 2:373-384.

4. Altirriba J., et al. (2010) Diabetologia 53:1406-1414.

[ IDENTIFICATION ]

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