跨膜蛋白27(TMEM27)重组蛋白(Mouse,小鼠) 

Recombinant Transmembrane Protein 27 (TMEM27)

NX17; Collectrin

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ile12~Leu222

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Kidney, Pancreas.

Subcellular Location: Membrane; Single-pass type I membrane protein.

Purity: >80%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 53.7kDa

Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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