跨膜蛋白27(TMEM27)重组蛋白(Rat,大鼠)

Recombinant Transmembrane Protein 27 (TMEM27)

NX17; Collectrin

[ PROPERTIES ]

Accession: Q9ESG3

Host: E.coli

Residues: Glu15~Pro141

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Membrane; Single-passType I Membrane Protein.

Purity: >90% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 18.2kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.ELCRPD AENAFKVRLS IKAALGDKAY VWDTDEEYLF RAMVAFSMRK VPNREGTEISHVLLCNVTQR VSFWFVVTDP LKNHTLPAAE VQSAIRMNRN RINSAFFLDDHTLEFLKIPSTLAPPMDPSV P

[ IDENTIFICATION ]

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