35kDa核孔蛋白(NUP35)重组蛋白(Human,人)

Recombinant Nucleoporin 35kDa (NUP35)

MP44; NP44; Mitotic phosphoprotein 44; Nuclear pore complex protein Nup53

[ PROPERTIES ]

Host: E.coli

Residues: Thr132~Lys310 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Nucleus, nuclear porecomplex. Nucleus membrane; Peripheralmembrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 23.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGQSMFSPA SIGQPRKTTLSPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL KHVMSNTGNWMHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME SSDRCALSSP SLAFTPPIKTLGTPTQPGST PRISTMRPLA TAYKASTSDY QVISDRQTPK

[ IDENTIFICATION ]

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