信号素4D(SEMA4D)重组蛋白(Human,人) 

Recombinant Semaphorin 4D (SEMA4D)

CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Phe502~Phe636

Tags: N-terminal His Tag

Tissue Specificity: Brain Intestine.

Subcellular Location: Cell membrane; Single-pass type I membrane protein.

Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 16.2kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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