基质金属蛋白酶13(MMP13)重组蛋白(Rat,大鼠2) 

Recombinant Matrix Metalloproteinase 13 (MMP13)

CLG3; Collagenase 3

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asp280~Val455 ,with N-terminal His-Tag.

Tags: N-terminal His Tag

Subcellular Location: Secreted, extracellularspace, extracellular matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 22.0kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGS - D PALSLDAITS LRGETMIFKD RFFWRLHPQQVEPELFLTKSFWPELPNHVD AAYEHPSRDL MFIFRGRKFW ALNGYDIMEG YPRKISDLGF PKEVKRLSAAVHFEDTGKTL FFSGNHVWSY DDANQTMDKD YPRLIEEEFP GIGDKVDAVY EKNGYIYFFNGPIQFEYSIW SNRIV

[ IDENTIFICATION ]

[ REFERENCES ]

1. Quinn C.O., et al. (1990) J. Biol. Chem. 265:22342-22347.

2. Sasano Y., et al. (2002) J. Histochem. Cytochem. 50:325-332.

3. Shum J.K., et al. (2002) J. Biol. Chem. 277:42830-42840.

4. Nishio T., et al. (2003) J. Hepatol. 38:468-475.

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