绒毛蛋白1(VIL)重组蛋白(Human,人) 

Recombinant Villin (VIL)

Villin 1; VIL1

[ PROPERTIES ]

Host: E.coli

Residues: Met1~Lys320 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Ruffle. Microvillus. Filopodium tip.

Purity: >95% 

Traits: Freeze-dried powder

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 39.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MTKLSAQVKG SLNITTPGLQ

IWRIEAMQMV PVPSSTFGSF FDGDCYIILA IHKTASSLSY DIHYWIGQDS SLDEQGAAAI

YT TQM D D F L K GRAV Q H REV Q GNE SE A F R GY FK Q G LVI RK G GVAS GMK HVE

TNSYDVQRLL HVKGKRNVVA GEVEMSWKSF NRGDVFLLDL GKLIIQWNGP ESTRMERLRG

MTLAKEIRDQ ERGGRTYVGV VDGENELASP KLMEVMNHVL GKRRELKAAV PDTVVEPALK

AALKLYHVSD SEGNLVVREV ATRPLTQDLL SHEDCYILDQ GGLKIYVWKG KKANEQEKKG

AMSHALNFIK

[ IDENTIFICATION ]

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