线粒体铁蛋白(FTMT)重组蛋白(Mouse,小鼠) 

Recombinant Ferritin, Mitochondrial (FTMT)

MtF

[ PROPERTIES ]

Host: E.coli

Residues: Ser54~His229 ,with N-terminal His-Tag.

Subcellular Location: Mitochondrion.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: : Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 21.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF- SSQDSTR PSRVRQNFHP DSEAAINRQI NLELYASYVY LSMAYYFSRDDVALYNFSKY FLRQSLEERE HAEKLMKLQN QRGGRICLQD IKKPDKDDWE CGLRAMECALLLEKNVNQSL LDLHTLASEK GDPHLCDFLE THYLHEQVKS IKELGDHVHN LVTMGAPAAGLAEYLFDKH

[ REFERENCES ]

1. Nie G., et al. (2005) Blood 105:2161-2167.

2. Wilkinson J., et al. (2006) Biochem. J. 395:501-507.

3. Nie G., et al. (2006) Blood 108:2428-2434.

4. Iwasaki K., et al. (2007) J. Biol. Chem. 282:22335-22343

[ IDENTIFICATION ]

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