Ig和ITIM域蛋白T-细胞免疫受体(TIGIT)重组蛋白(Mouse,小鼠) 

Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

VSIG9; VSTM3; V-Set And Immunoglobulin Domain Containing 9; V-Set And Transmembrane Domain Containing 3

[ PROPERTIES ]

Host: E.coli

Residues: Gly28~Ser246 , with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Cell membrane; Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 27.7kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: 

The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37°C for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GTI DTKRNISAEE GGSVILQCHF

SSDTAEVTQV DWKQQDQLLA IYSVDLGWHV ASVFSDRVVP GPSLGLTFQS LTMNDTGEYF

CTYHTYPGGI YKGRIFLKVQ ESSDDRNGLA QFQTAPLGGT MAAVLGLICL MVTGVTVLAR

KDKSIRMHSI ESGLGRTEAE PQEWNLRSLS SPGSPVQTQT APAGPCGEQA EDDYADPQEY

FNVLSYRSLE SFIAVS

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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