肾脏脑蛋白(KIBRA)重组蛋白 (Mouse,小鼠) 

Recombinant Kidney And Brain Protein (KIBRA)

WWC1; WW Domain Containing Protein 1; WW,C2 And Coiled-Coil Domain Containing 1; HBeAg-binding protein 3

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Glu847~Met1094

Tags: N-terminal His Tag

Tissue Specificity: Mammary Gland, Mammary Tumor, Salivary Gland, Liver.

Subcellular Location: Cell membrane. Cell projection. Cytoplasm. Membrane. Nucleus.

Purity: >98% 

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 32.4kDa

Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. . Relative charge: The composition of amino acids may affects the charge of the protein.

3. . Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37oC for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition

[ SEQUENCE ]

[ IDENTIFICATION ]

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