血管生成素样蛋白2(ANGPTL2)重组蛋白(Mouse,小鼠) 

Recombinant Angiopoietin Like Protein 2 (ANGPTL2)

HARP; ARP2; Angiopoietin-Related Protein 2

[ PROPERTIES ]

Host: E.coli

Residues: Ser267~His493

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9R045

Subcellular Location: Secreted.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 30.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

STDK PSGPWRDCLQ ALEDGHSTSS IYLVKPENTN RLMQVWCDQR HDPGGWTVIQRR L D G S V N F F RNW E T Y K Q G F GNI D G E YW L G LE NI YW LT N Q GNY K L LV TM EDW S G RK V FA E YA S F R LE P E S EY Y K L R L G RY HG NA G DS F TWHN G K Q F T T L DRDHDVYTGNC AHYQKGGWWY NACAHSNLNG VWYRGGHYRS RYQDGVYWAEFRGGSYSLKK VVMMIRPNPN TFH

[ REFERENCES ]

1. Kim I., et al. (1999) J. Biol. Chem. 274:26523-26528.

2. Tabata M., et al. (2009) Cell Metab. 10:178-188.

3. Aoi J., et al. (2011) Cancer Res. 71:7502-7512.

4. Kanda A., et al. (2012) Lab. Invest. 92:1553-1563.

[ IDENTIFICATION ]

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